Things to know for biotech quiz

REs

-used to cut DNA fragment from a sequence.

-Sticky and blunt are the two type of cuts that REs can cut.

Vector Cloning

-target gene is inserted into a recombinant DNA and reinserted back into the bacterium where transformation occurs.

PCR

-Taq polymerase is used because of its ability to survive in heat.

-target sequence is obtained at the 3^rd cycle

Gel electrophoresis

-used to locate desired gene base on DNA fragment size.

-DNA is negatively charged and the larger fragment stays on the top while the smaller fragment travels further.

RFLP

-used to detect certain differences in DNA sequence.

DNA sequencing

-adds 0.01% of ddNTP to create fragment of different length.

-used to determine the DNA sequence.