My Biology blog
Tuesday, 18 October 2011
Things to know for biotech quiz
-used to cut DNA fragment from a sequence.
-Sticky and blunt are the two type of cuts that REs can cut.
-target gene is inserted into a recombinant DNA and reinserted back into the bacterium where transformation occurs.
-Taq polymerase is used because of its ability to survive in heat.
-target sequence is obtained at the 3
-used to locate desired gene base on DNA fragment size.
-DNA is negatively charged and the larger fragment stays on the top while the smaller fragment travels further.
-used to detect certain differences in DNA sequence.
-adds 0.01% of ddNTP to create fragment of different length.
-used to determine the DNA sequence.
Share to Twitter
Share to Facebook
Share to Pinterest
Post a Comment
Post Comments (Atom)