Tuesday, 18 October 2011

Things to know for biotech quiz

-used to cut DNA fragment from a sequence.
-Sticky and blunt are the two type of cuts that REs can cut.
Vector Cloning

-target gene is inserted into a recombinant DNA and reinserted back into the bacterium where transformation occurs.

-Taq polymerase is used because of its ability to survive in heat.
-target sequence is obtained at the 3rd cycle
Gel electrophoresis

-used to locate desired gene base on DNA fragment size.
-DNA is negatively charged and the larger fragment stays on the top while the smaller fragment travels further.

-used to detect certain differences in DNA sequence.
DNA sequencing

-adds 0.01% of ddNTP to create fragment of different length.
-used to determine the DNA sequence.

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